Sequence-Specific Recognition of Double-Stranded DNA by Peptide Nucleic Acid Forming Double-Duplex Invasion Complex

Peptide nucleic acid (PNA) is an analog of natural acids, where the sugar-phosphate backbone DNA replaced by electrostatically neutral N-(2-aminoethyl)glycine backbone. This unique peptide-based enables PNAs to form a very stable duplex with complementary acids via Watson–Crick base pairing since there no electrostatic repulsion between PNA and DNA·RNA. With this high affinity, have been used in wide range fields, from biological applications such as gene targeting, engineering probe sensor developments. In addition single-stranded DNA, can also recognize double-stranded (dsDNA) through formation double-duplex invasion complex. hard achieve other artificial expected be promising method dsDNA cellula or vivo does not require prior denaturation dsDNA. paper, we provide basic knowledge mainly focus on research invasion.